StepReg - Stepwise Regression Analysis

Stepwise regression is a statistical technique used for model selection. This package streamlines stepwise regression analysis by supporting multiple regression types(linear, Cox, logistic, Poisson, Gamma, and negative binomial), incorporating popular selection strategies(forward, backward, bidirectional, and subset), and offering essential metrics. It enables users to apply multiple selection strategies and metrics in a single function call, visualize variable selection processes, and export results in various formats. StepReg offers a data-splitting option to address potential issues with invalid statistical inference and a randomized forward selection option to avoid overfitting. We validated StepReg's accuracy using public datasets within the SAS software environment. For an interactive web interface, users can install the companion 'StepRegShiny' package.

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7.41 score 2 stars 2 dependents 46 scripts 1.2k downloads

TmCalculator - Extending Nucleic Acid Melting Temperature Analysis from Sequence-Level Computation to Genome-Wide Thermodynamic Profiling

Accurate calculation of nucleic acid melting temperature (Tm) is fundamental to many molecular biology applications, and this software scales Tm analysis from individual sequences to genome‑wide thermodynamic profiling. This package extends Tm analysis from simple sequence level computation to comprehensive genome-wide thermodynamic profiling. It takes multiple input formats including sequence strings, FASTA files, genomic coordinates. The implementation provides three Tm calculation methods: the Wallace rule (Thein & Wallace, 1986), empirical GC‑content formulas (Marmur, 1962; Schildkraut, 2010; Wetmur, 1991; Untergasser, 2012; von Ahsen, 2001), and nearest‑neighbor thermodynamics (Breslauer, 1986; Sugimoto, 1996; Allawi, 1998; SantaLucia, 2004; Freier, 1986; Xia, 1998; Chen, 2012; Bommarito, 2000; Turner, 2010; Sugimoto, 1995; Allawi, 1997; SantaLucia, 2005). Corrections are supported for salt ions (SantaLucia, 1996, 1998; Owczarzy, 2004, 2008) and for chemical conditions such as dimethyl sulfoxide and formamide. This package returns result as a GRanges object for interoperability with Bioconductor workflows and downstream multi-omics analyses (e.g., ATAC‑seq, ChIP‑seq, RNA‑seq, QTL/GWAS). Data-level integration reconciles Tm windows with external multi-omics GRanges objects through overlap, nearest-feature, windowed-count, and binned-average strategies, returning a single unified GRanges object ready for downstream analysis. Visualization-level integration renders multiple feature layers as independent concentric tracks on a shared genomic axis, each retaining its native coordinate resolution. Group comparison supports Wilcoxon rank-sum and Student's t-tests with multiple available correction methods for contrasting Tm and other features across region classes.

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5.27 score 4 stars 1 dependents 52 scripts 735 downloads

StepRegShiny - Graphical User Interface for 'StepReg'

A web-based 'shiny' interface for the 'StepReg' package enables stepwise regression analysis across linear, generalized linear (including logistic, Poisson, Gamma, and negative binomial), and Cox models. It supports forward, backward, bidirectional, and best-subset selection under a range of criteria. The package also supports stepwise regression to multivariate settings, allowing multiple dependent variables to be modeled simultaneously. Users can explore and combine multiple selection strategies and criteria to optimize model selection. For enhanced robustness, the package offers optional randomized forward selection to reduce overfitting, and a data-splitting workflow for more reliable post-selection inference. Additional features include logging and visualization of the selection process, as well as the ability to export results in common formats.

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4.00 score 2 scripts 188 downloads

ZetaSuite - Analyze High-Dimensional High-Throughput Dataset and Quality Control Single-Cell RNA-Seq

The advent of genomic technologies has enabled the generation of two-dimensional or even multi-dimensional high-throughput data, e.g., monitoring multiple changes in gene expression in genome-wide siRNA screens across many different cell types (E Robert McDonald 3rd (2017) <doi: 10.1016/j.cell.2017.07.005> and Tsherniak A (2017) <doi: 10.1016/j.cell.2017.06.010>) or single cell transcriptomics under different experimental conditions. We found that simple computational methods based on a single statistical criterion is no longer adequate for analyzing such multi-dimensional data. We herein introduce 'ZetaSuite', a statistical package initially designed to score hits from two-dimensional RNAi screens.We also illustrate a unique utility of 'ZetaSuite' in analyzing single cell transcriptomics to differentiate rare cells from damaged ones (Vento-Tormo R (2018) <doi: 10.1038/s41586-018-0698-6>). In 'ZetaSuite', we have the following steps: QC of input datasets, normalization using Z-transformation, Zeta score calculation and hits selection based on defined Screen Strength.

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4.00 score 5 scripts 518 downloads

JM4QTN - Joint Mapping for Quantitative Trait Loci

A comprehensive computational framework for joint mapping, developed by Junhui Li (2016; doi:10.11841/j.issn.1007-4333.2016.06.002), supports quantitative trait locus detection in structured genetic populations. It integrates robust phenotype summarization, computes genotype probabilities, and imputes missing markers for association and linkage mapping. Empirical significance thresholds are estimated via permutation testing coupled with stepwise regression. The framework enables genome-wide scans under both univariate and multivariate trait models, streamlining the discovery of complex genetic architectures.

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3.18 score 73 downloads

ProbeDeveloper - Develop Hybridization Probes

Hybridization probes for target sequences can be made based on melting temperature value calculated by R package 'TmCalculator' <https://CRAN.R-project.org/package=TmCalculator> and methods extended from Beliveau, B. J.,(2018) <doi:10.1073/pnas.1714530115>, and those hybridization probes can be used to capture specific target regions in fluorescence in situ hybridization and next generation sequence experiments.

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2.70 score 170 downloads

JM4QTN - Joint Mapping for Quantitative Trait Loci

A comprehensive computational framework for joint mapping, developed by Li (2016) <doi:10.11841/j.issn.1007-4333.2016.06.002>, supports quantitative trait locus detection in structured genetic populations. It integrates robust phenotype summarization, computes genotype probabilities, and imputes missing markers for association and linkage mapping. Empirical significance thresholds are estimated via permutation testing coupled with stepwise regression. The framework enables genome-wide scans under both univariate and multivariate trait models, streamlining the discovery of complex genetic architectures.

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1.70 score

gtWAS - Genome and Transcriptome Wide Association Study

Quantitative trait loci mapping and genome wide association analysis are used to find candidate molecular marker or region associated with phenotype based on linkage analysis and linkage disequilibrium. Gene expression quantitative trait loci mapping is used to find candidate molecular marker or region associated with gene expression. In this package, we applied the method in Liu W. (2011) <doi:10.1007/s00122-011-1631-7> and Gusev A. (2016) <doi:10.1038/ng.3506> to genome and transcriptome wide association study, which is aimed at revealing the association relationship between phenotype and molecular markers, expression levels, molecular markers nested within different related expression effect and expression effect nested within different related molecular marker effect. F test based on full and reduced model are performed to obtain p value or likelihood ratio statistic. The best linear model can be obtained by stepwise regression analysis.

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1.00 score 1 stars 7 scripts 488 downloads